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. 2021 May 18;13(10):2461. doi: 10.3390/cancers13102461

Figure 3.

Figure 3

Simulation and experimental validation of DARPin penetration into tumor spheroids. (A–D) Experimental data vs. simulation data using four different diffusion coefficients. The y-axis is the percentage of penetration (i.e., of total distance) from the outside towards the core. The spheroid radius was set at 150 µm for simulated data, which was the average radius; n = 5 spheroids per time point. The distinct conditions were Ec1 at 500 nM (A), Ec1 at 100 nM (B), 9_26 at 500 nM (C) and 9_26 at 100 nM (D). (E) Confocal microscopy images of cleared BT-474 spheroids at several time points of incubation of DARPins at 500 nM or after 4 h incubation at 100 nM. Scale bars are 100 µm. (F) Quantification of the time dependency of the penetration depth of DARPins Ec1 and 9_26 in BT-474 spheroids; n = 5. (G) Quantification of concentration dependency of DARPin penetration into BT-474 spheroids; n = 3, error bars correspond the standard error of the mean (SEM). **** p < 0.0001 based on a two-way ANOVA.