Table 1.
Formulation and proximate composition of the experimental diets (Control, and test diets introducing 4.5, 7.5, or 10.5% of BSFI respectively–BSFI4.5, BSFI7.5, and BSFI10.5).
| Raw Materials | Dietary Treatments | |||
|---|---|---|---|---|
| Control (g/100 g) | BSFI4.5 (g/100 g) | BSFI7.5 (g/100 g) | BSFI10.5 (g/100 g) | |
| Black Soldier Fly Ingredient a | 0.00 | 4.50 | 7.50 | 10.50 |
| Wheat, flour | 35.25 | 32.74 | 31.74 | 31.59 |
| Soybean meal b | 30.50 | 32.50 | 33.25 | 33.50 |
| Fish meal c | 15.00 | 10.50 | 7.50 | 4.50 |
| Poultry by-product meal d | 7.00 | 7.00 | 7.00 | 7.00 |
| Sepiolite e | 1.50 | 1.49 | 1.21 | 0.60 |
| Fish oil f | 2.84 | 2.90 | 2.84 | 2.96 |
| Squid meal g | 2.50 | 2.50 | 2.65 | 2.65 |
| Soy lecithin h | 1.50 | 1.57 | 1.67 | 1.78 |
| Limestone i | 0.60 | 0.60 | 0.70 | 0.80 |
| Vit min premix j | 0.50 | 0.50 | 0.50 | 0.50 |
| Monocalcium phosphate k | 0.65 | 0.97 | 1.17 | 1.30 |
| Cholesterol, feed grade l | 0.10 | 0.10 | 0.10 | 0.10 |
| Methionine m | 0.05 | 0.12 | 0.16 | 0.21 |
| Gelatine n | 2.00 | 2.00 | 2.00 | 2.00 |
| Astaxanthin premix o | 0.01 | 0.01 | 0.01 | 0.01 |
| Total | 100.00 | 100.00 | 100.00 | 100.00 |
| Parameter | Proximate Analysis | |||
| DM% | 100 | 100 | 100 | 100 |
| Ash% | 7.00 | 7.21 | 7.42 | 7.53 |
| CP% | 40.40 | 40.30 | 40.10 | 39.70 |
| Lipid% | 9.63 | 9.72 | 9.74 | 9.96 |
| Fiber% | 1.82 | 1.86 | 1.87 | 1.88 |
| LOA (18:2n-6)% | 2.03 | 2.08 | 2.15 | 2.23 |
| LNA (18:3n3)% | 0.16 | 0.16 | 0.17 | 0.17 |
| ARA (20:4n-6)% | 0.08 | 0.08 | 0.07 | 0.07 |
| EPA (20:5n-3)% | 0.36 | 0.33 | 0.30 | 0.28 |
| DHA (22:6n-3)% | 0.95 | 0.94 | 0.90 | 0.92 |
| Total n-3% | 1.47 | 1.43 | 1.37 | 1.37 |
| Total n-6% | 2.10 | 2.15 | 2.22 | 2.30 |
| n-3:n-6 | 0.79 | 0.75 | 0.69 | 0.67 |
| Total phospholipid% | 2.56 | 2.51 | 2.51 | 2.51 |
| Cholesterol% | 0.18 | 0.19 | 0.19 | 0.19 |
| Astaxanthin (mg/kg) | 9.03 | 9.00 | 8.99 | 8.98 |
| Arginine% | 2.60 | 2.52 | 2.47 | 2.40 |
| Histidine% | 1.03 | 1.03 | 1.02 | 1.01 |
| Isoleucine% | 1.82 | 1.77 | 1.73 | 1.68 |
| Leucine% | 3.05 | 2.97 | 2.91 | 2.82 |
| Lysine% | 2.70 | 2.57 | 2.48 | 2.36 |
| Methionine% | 0.92 | 0.91 | 0.90 | 0.90 |
| Phenylalanine% | 1.88 | 1.85 | 1.82 | 1.78 |
| Threonine% | 1.63 | 1.59 | 1.56 | 1.52 |
| Tryptophan% | 0.50 | 0.63 | 0.71 | 0.79 |
| Valine% | 2.07 | 2.04 | 2.01 | 1.96 |
| Ca% | 1.49 | 1.42 | 1.42 | 1.40 |
| Available P% | 1.11 | 1.12 | 1.12 | 1.11 |
a InnovaFeed; b 48% CP 200mn, (VDS); c 70% CP, Norvik low temperature (SEah International); d low ash, 62% CP (Cagemax); e <100 mesh, (Poortershaven); f Tuna oil, crude 031508/01 (VDS); g (Seah International); h Bergapur de-oiled, (Speerstra); i Calcium Carbonate S 48/49, (VDS); j Crustocean TV21/10, (VDS); k (VDS); l XG, (Dishan); m MERA Met Ca, (NOVUS) n (VDS); o (VDS).