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. 2021 May 18;13(10):2462. doi: 10.3390/cancers13102462

Figure 1.

Figure 1

Generation and validation of KCCux1 mice. (A) Breeding scheme for the generation of KrasLSL-G12D/+; Ptf1aCre/+; CMV-Cux1+/− mice (p200 CUX1). KrasLSL-G12D/+; CMV-Cux1+/− mice were mated with Ptf1aCre/+ mice to generate Kras-activated KrasLSL-G12D/+; Ptf1aCre/+ and KrasLSL-G12D/+; Ptf1aCre/+; CMV-Cux1+/− animals. (B) Representative immunohistochemistry (top) of CUX1 in pancreatic section obtained from 3-month-old WT (i), Cux1 (ii), KC (iii) and KCCux1 (iv), as well as 15-month-old KC (v) and KCCux1 (vi) animals. Original magnification: 200×. Scale bar, 50 µm. Quantification (below) of Cux1 intensity in pancreatic ducts of 3-month-old WT (n = 5) and Cux1 (n = 5) mice and in mPanIN areas of 3-and 15-month-old KC (n = 8) and KCCux1 (n = 7) mice. Immunohistochemistry intensity score: + weak, ++ moderate, +++ strong.