Figure 6.

p110 CUX1 potentiates proliferation via upregulation of ADAM17 and increased EGFR activation in KRAS-mutant pancreatic cell lines. (A,B) Western blot analysis of whole protein cell lysates from stable transduced mPanIn, Panc-1 and hHPNE-KRas cells. Actin served as control. (A) Overexpression of p110 potentiated the protein levels of the cell proliferation markers pERK, CDK4 and CCND1 compared to empty vector (LV) control cells. Phosphorylated ERK and total ERK were determined using specific antibodies. (B) Compared to empty vector (LV) control cells, overexpression of p110 was associated with increased protein levels of total EGFR and phosphorylated EGFR (Y1068), as well as ADAM17, an EGFR ligand sheddase. Phosphorylated EGFR and total EGFR were determined using specific antibodies. The uncropped Western Blot images can be found in Figure S9 and the densitometry analysis can be found in Figure S10.