Figure 4.

(A) Schematic depiction of cell culture experimental steps. The PC/TPE-hybrid–glass device is initially coated with type I collagen. Excess collagen is flushed out and followed by HUVEC seeding. The chip is temporarily flipped during cell seeding to ensure cell adhesion to both sides of the channel. (B) Micrographs acquired using phase contrast microscopy show cell attachment and progression of the cell culture from day 1 to day 3 for both substrates (left). (C) Fluorescence microscopy images from the live/dead staining (Hoechst/FDA/PI) on day 3 (left: composite image; right: individual color channels). Scale bars: 100 µm. N = 3.