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. 2021 May 19;9(5):1089. doi: 10.3390/microorganisms9051089

Figure 2.

Figure 2

Analysis of fusion between autophagosomes and glycosomes or lysosomes. (A) Axenic promastigotes of Leishmania expressing GFP-ATG8 and RFP-SQL were treated or not with 17-AAG (500 nM) and imaged by fluorescence microscopy. (B) Quantification of autophagosome-glycosome colocalization after treatment of Leishmania with 17-AAG. (C) Axenic promastigotes of Leishmania expressing ATG8-GFP and proCPB-RFP were treated or not with 17-AAG (500 nM) and imaged by fluorescence microscopy. (D) Quantification of Leishmania autophagosome-lysosome colocalization after treatment with 17-AAG. Bars represent medians ± SD from one out of three independent experiments (Unpaired t test, *** p = 0.0006, * p = 0.0197).