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Ubiquitylated protein profiles of WT, Δatg5 and Δatg5::ATG5 after 17-AAG or MG132 treatment. WT, Δatg5 and Δatg5::ATG5 parasites were treated with 17-AAG (500 nM) or MG132 (3 µM) for 48 h. Protein extracts were electrophoresed on a 12% gel, blotted and probed with an FK2 anti-ubiquitin antibody. EF1α was used as loading control.
