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Effect of 17-AAG treatment on WT, Δatg5 and Δatg5::ATG5 protein aggregate formation. Culture aliquots from WT, Δatg5 and Δatg5::ATG5 parasites treated with 17-AAG (500 nM) or MG132 (3 µM) for 24 h were withdrawn and analyzed to determine quantities of insoluble protein aggregates by cell lysis and centrifugation. Protein aggregates were subjected to SDS-PAGE followed by silver staining. One experiment is representative of two independent experiments.
