Table 1.
Remaining percentage of the original vg amount of rAAVs after the distinct process steps.
| Purification Strategy | Purification Step | TFF (%) | SD (%) | PEG Precipitate (%) | SD (%) | Cell Pellet Lysate (%) | SD (%) |
|---|---|---|---|---|---|---|---|
| HIC-CEX-AEX | HIC | 23 | 6 | 3 | - | 0.3 | - |
| CEX | 15 | 7 | 0.3 | - | 0.2 | - | |
| AEX | 5 | 2 | ND | ND | ND | ND | |
| CEX-AEX | CEX | ND | ND | ND | ND | 65 | 13 |
| AEX | ND | ND | ND | ND | 59 | 24 | |
| AC-AEX | AC | 89 | 21 | 69 | 24 | 59 | 12 |
| AEX | 32 | 11 | 44 | 21 | 37 | 23 |
AC, affinity chromatography; AEX, anion exchange chromatography; CEX, cation exchange chromatography; HIC, hydrophobic interaction chromatography; ND, not done; PEG, polyethylene glycol; rAAV, recombinant adeno-associated virus; SD, standard deviation; TFF, tangential flow filtration.