Table 2.
Amount of empty capsids in the fractions containing full rAAVs determined by analytical AEX.
| Purification Strategy | Number of Preparations | Residual Amount of Empty Capsids in Full rAAV Fractions (%) | |
|---|---|---|---|
| Entire Peak | Peak Maximum | ||
| CEX-AEX(E/F) linear | 1 | 27 | 21 |
| 2 | 31 | 23 | |
| CEX-AEX(E/F) steps | 1 | 17 | 8 |
| 2 | 17 | 12 | |
| 3 | 20 | 9 | |
| AC-AEX(E/F) linear | 1 | 23 | 15 |
| 2 | 40 | 28 | |
| AC-AEX(E/F) steps | 1 | 20 | 13 |
AC, affinity chromatography; AEX, anion exchange chromatography; CEX, cation exchange chromatography; rAAV, recombinant adeno-associated virus.