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Remaining percentage of the original vg amount of rAAV after each different purification step.
| Purification Strategy | Number of Preparations | Purification Step | Yield (%) |
|---|---|---|---|
| CEX-AEX(E/F) linear | 1: Cell pellet | CEX | 64 |
| AEX | 70 | ||
| 2: PEG pellet | CEX | 80 | |
| AEX | 31 | ||
| CEX-AEX(E/F) steps | 1: Cell pellet | CEX | 86 |
| AEX | 70 | ||
| 2: Cell pellet | CEX | 55 | |
| AEX | 78 | ||
| 3: PEG pellet | CEX | 92 | |
| AEX | 63 |
AEX, anion exchange chromatography; CEX, cation exchange chromatography; PEG, polyethylene glycol.
