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. 2021 May 18;22(10):5327. doi: 10.3390/ijms22105327

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enhanced telomerase expression amplifies mitochondrial genes and improves mitochondrial potential. (A) JC-1 staining in day-8 blastocysts from the control, D-galactose, and hTERT-treated groups (20 per each group) and Bar = 100 µm. (B) TUNEL assay was performed to detect apoptotic cells. The D-galactose group had significantly more TUNEL positive cells than the control and TERT plasmid injected groups (n = 20 per group) and Bar = 100 µm. (C) The relative mRNA expressions of NRF-1 and TFA in bovine day-8 blastocysts. The expression of both the genes were significantly reduced in D-gal group as compared to control and Plasmid groups. The data shown here as a mean ± S.E.M. N.S, not significant, * = p < 0.05, and ** = p < 0.01.