. Author manuscript; available in PMC: 2021 May 27.

Published in final edited form as: Angew Chem Int Ed Engl. 2020 Jan 28;59(11):4511–4518. doi: 10.1002/anie.201914576

Table 1.

Photophysical and Binding Properties of Fluorophore-Broccoli Complexes

RNA-Fluorophore	Excitation (nm)	Emission (nm)	Extinction Coefficient (M⁻¹, cm⁻¹)^[a]	Quantum Yield	Brightness^[b]	K_D (nM)
DFHBI-1T	428	500	35,400	0.00041	0.121	N/A
Broccoli- DFHBI-1T	470	505	28,900	0.414	100	305±39
BI	425	500	42,500	0.00048	0.171	N/A
Broccoli- BI	470	505	33,600	0.669	188	51±5

^[a].

Extinction coefficients were all measured in buffer containing 40 mM HEPES pH 7.4, 100 mM KCl, 1 mM MgCl₂. Experiments were performed at 25°C in order to compare with previous studies.^[1]

^[b].

Brightness (extinction coefficient × quantum yield) is relative to Broccoli-DFHBI-1T.