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. 2021 May 13;15:541676. doi: 10.3389/fncir.2021.541676

TABLE 1.

Materials.

Name of material/Equipment Company Catalog number Comments/Description
0.9% Saline Sodium Chloride Injection Bag Baxter Healthcare Corporation 288-0006AA SURGERY. To safely flush tissue other than the brain
Hanks’ Balanced Salt solution (HBSS) MilliporeSigma Canada Co. 55021C SURGERY. Solution applied topically to the brain during surgery, to keep the brain from drying
Sugi cellulose absorbent triangles Kettenbach GmbH & Co. KG, Germany 001911 SURGERY. To absorb excess solutions or blood from tissues
Dowsil Silicone sealant Dow Corning 3140 90ML MIL-A-46146 SURGERY. For a rigid hydrophobic silicone chamber for holding HBSS
Ethilon Sutures 5-0 Ethicon-Johnson & Johnson 661H (nylon monofilament, FS-2, 45 cm strands) RECOVERY SURGERY. To connect the skin flaps after the recovery surgery
Polysporin Complete ointment Johnson & Johnson 60245-43775 RECOVERY SURGERY. To avoid infections during post-op recovery period; it contains three antibiotics plus lidocaine hydrochloride
Syringe Priming Kit Chromatographic Specialties Inc. HPRMKIT VIRAL MICROINJECTIONS. Indispensable to load up the virus into the syringe
Syringe 10 μL removable needle Hamilton 701RN VIRAL MICROINJECTIONS. To hold the virus
Glass micropipettes tubes World Precision Instruments Inc. 18100-3 (3IN BOROSIL GL 1.0 MM OD) VIRAL MICROINJECTIONS. They will need to be pulled into shape
PHD ULTRATM Syringe Infuse/Withdraw Programmable Micropump Harvard Apparatus 70-3007 VIRAL MICROINJECTIONS. For pumping the viral solution through the micropipette at an optimal low rate
Master9 Programmable Pulse Stimulator A.M.P.I. STIMULATION. For timing the sensory stimuli
Electrical constant current stimulator World Precision Instruments Inc. A365 STIMULATION. For generating electrical current sent to the piezoelectric actuator
Piezoelectric actuator Piezo Systems Inc. PSI-5A4E, Y-poled, double quick-mount bender STIMULATION. For whisker stimulation
VDAQ 3001 Optical Imaging Inc. OPTICAL IMAGING. For data acquisition
Camera lens Nikon 1987 (60 MM F/2.8 D-AF) OPTICAL IMAGING. Optimal for wide-field OI-IS
Camera zoom lens Edmund Optics VZM1000i OPTICAL IMAGING. Optimal for imaging at high-magnification
LED 530 nm Mightex BLS-LCS-0530-15-22 OPTICAL IMAGING. LED for Optical Imaging
Electrode for acute insertion and recordings NeuroNexus A1 × 32-50-177-A32 NEUROPHYSIOLOGY. Electrode characteristics customizable to the experimental needs
Optogenetic virus Neurophotonics Centre – Molecular Tools Platform – Ulaval Viral vector selected according to the scientific question OPTOGENETICS. The viral vector to be injected into the brain
LED 470 or 595 nm ThorLabs M470F3, M595F2 OPTOGENETICS. LED for optogenetic photostimulation
Multimode optical fiber ThorLabs FPC-1000-37-02SMA OPTOGENETICS. Multimode Fiber Patchcord with 0.37 NA, 1000 μm core diameter and SMA connectors
DiI Vybrant cell-labeling solution Life Technologies V22885 HISTOLOGY. For marking the track of the electrode
Cytochrome C from bovine heart Sigma Aldrich C3131-10MG HISTOLOGY. For cytochrome oxidase staining
DAB (3,3′-Diaminobenzidine tetrahydrochloride) Sigma Aldrich D5905 HISTOLOGY. For cytochrome oxidase staining
DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific D3571 HISTOLOGY. For DAPI fluorescence counterstaining