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. 2021 May 12;6(20):13153–13160. doi: 10.1021/acsomega.1c00976

Figure 4.

Figure 4

(a) Sequences and modifications of NBOP-17Dz-DQ, DEACM-17Dz-DQ, and 17S-FQ used in the cellular study. C9 is a Spacer 9 modification; F corresponds to fluorescein; Q refers to Dabyl or Iowa Black Quencher. (b and c) Confocal microscopy images (b) and flow cytometry analyses (c) of HeLa cells transfected with 17S-FQ and each of the DNAzymes including PS-17Dz-DQ, NBOP-17Dz-DQ, and DEACM-17Dz-DQ, before and after light irradiation. Fluorescence was only observed upon the cleavage of 17S-FQ by active DNAzyme sensors. Scale bar = 100 μm. For error bars, n = 3. Conditions for light irradiation: 365 nm at 26 mW/cm2 for 5 min and 470 nm at 13 mW/cm2 for 10 min.