Table 1. Hub Proteins of Networks Present Only in mPS1 Exosomesa.
| gene name | protein description | # nodes | functions in the brain, AD, related |
|---|---|---|---|
| COL5A3 | collagen type V α-3 chain | 6 | fibril-forming collagen53 |
| COL6A2 | collagen type VI α-2 chain | 3 | beaded filament collagen53 |
| marker present in cerebral amyloid angiopathy54 | |||
| COL6A3 | collagen type VI α-3 chain | 5 | beaded filament collagen53 |
| related to dystonia, alcohol dependence, and congenital muscular dystrophy55−57 | |||
| COL8A1 | collagen type VIII α-1 chain | 7 | network-forming collagen53 |
| involved in vessel wall integrity53 | |||
| COL14A1 | collagen type XIV α-1 chain | 4 | fibril-associated collagen with interrupted triple helices53 |
| DCN | decorin | 4 | role in collagen fibril assembly60 |
| stimulatory effect in autophagy and inflammation61 | |||
| TLL1 | tolloid-like 1, metalloendopeptidase | 4 | metalloprotease that cleaves fibrillar procollagens I, II, III, V, and VI58 |
| role in neurogenesis58 | |||
| PCOLCE | procollagen endopeptidase enhancer 1 | 4 | enhances procollagen C-proteinase activity that cleaves type I procollagen C-propeptide59 |
| PRSS23 | serine protease 23 | 4 | vascular protease62 |
| IGFBP7 | insulin-like growth factor-binding protein 7 | 3 | attenuates function of ILPs63 |
| upregulated in human AD brain63 | |||
| inhibition of IGFBP7 in AD mice restores memory impairment and associative learning behavior63 | |||
| biomarker for AD63 | |||
| APOA2 | apolipoprotein A2 | 3 | role in lipid metabolism64 |
| associated with lifespan and cognitive function64 |
a
Proteins identified only in the mutant PS1 exosomes are listed by gene name, number of interacting proteins terms “nodes”, and description of the protein function. All proteins were identified with FDR less than 1% (see Experimental Procedures).