Fig 3. Independent Foxa2+ progenitors contribute to the ventricles and outflow tract.

(A) Schematics of the Foxa2^{nGPF-CreERT2/+}; R26R^tdTomato/+ allele [16]. (B) Pulse-labelling of the Foxa2-expressing cells and their descendants, from stage (i) E6+21h, (ii) E7+7h by administration of tamoxifen to Foxa2^{nGPF-CreERT2/+}; R26R^tdTomato/+ mice (0.08 mg/body weight via oral gavage). Cell descendants in the heart analysed at E12.5. (C) Representative hearts resulting from the administration of tamoxifen at different time points in Foxa2^{nGPF-CreERT2/+}; R26R^tdTomato/+ immunostained for cTnnT to reveal the cardiomyocytes (blue). Views are ventral. Single epicardial cells are also labelled in (iii). (D, E) Summary of all hearts analysed. The contribution of the Foxa2-expressing cells to the different compartments of the heart is quantified by measuring the proportion of tdTomato-positive myocardium. Numbers in brackets in (E) represent the number of litters assessed. Error bars are SD. Scale bar: 200 μm. The data underlying (D, E) can be found in S3 Source Data. cTnnT, cardiac troponinin T; LA, left atria; LV, left ventricle; OFT, outflow tract; RA, right atria; RV, right ventricle.