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. 2021 May 17;17(5):e1009561. doi: 10.1371/journal.pgen.1009561

Fig 4. DEM1 and DEM2 are required for normal seed production in Arabidopsis.

Fig 4

(A-C) Plants harboring only one wild-type DEM1 allele (DEM1/dem1 dem2/dem2) in both the Col-0 and Ws-0 genetic backgrounds exhibited significant ovule abortion. (A) Comparison of wild-type (WT) and DEM1/dem1 dem2/dem2 siliques of Col-0 genetic background. Note the occurrence of degenerated ovules in DEM1/dem1 dem2/dem2 siliques compared to the appearance of normal seeds in WT siliques. (B-C) Percentage of aborted ovules in self-fertilized WT and dem genotypes for Col-0 (B) and Ws-0 (C) genetic backgrounds. At least 200 ovules were assayed, involving at least three separate plants for each genotype. X-axis of (B) and (C) represent the copy number of DEM1 and DEM2 wild-type alleles in each genotype. No dem1/dem1 DEM2/dem2 plants were recovered in the Ws-0 genetic background; n.d., not determined.