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. 2021 May 17;17(5):e1009561. doi: 10.1371/journal.pgen.1009561

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© 2021 Lee et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A-D) Defective female gametophytes produced in DEM1/dem1 dem2/dem2 plants (Ws-0 genetic background). Developing ovules were observed as cleared whole mounts. (A-B) Four cell embryo (white arrowhead) and mononucleate embryo sac (black arrowhead) in two adjacent ovules. (C-D) Two cell embryo (white arrowhead) and di-nucleate embryo sac (black arrowheads) in two adjacent ovules. (E) Percentage of mononucleate and di-nucleate embryo sacs in self-fertilized wild-type and DEM1/dem1 dem2/dem2 plants of ecotype Ws-0. At least 200 ovules were assayed, involving at least three separate plants for each genotype. X-axis represents the copy number of DEM1 and DEM2 wild-type alleles in each genotype.