Table 1. Transmission of gametes depends on the dosage of functional DEM alleles in both the gametes and the plant that undergoes meiosis to produce the gametes.
Segregation data from crosses involving dem mutations in the two genetic backgrounds (Col-0 and Ws-0) were analyzed separately and together for significance. The expected segregation of genotypes assuming equal transmission of wild-type DEM and mutant dem alleles is listed in brackets. N, number of progeny scored; P, probability for a chi-square distribution with three degrees of freedom and an expected segregation ratio of 1:1:1:1, or with one degree of freedom and an expected segregation ratio of 1:1. For some crosses, chi-square probabilities were also determined for observed and expected segregation ratios for just dem1 DEM2 versus DEM1 DEM2 gametes, and these probabilities are listed in the third row of the table for these crosses.
| Cross (♀ x ♂) |
N | Genotype of gametes transmitted | P | |||
|---|---|---|---|---|---|---|
|
dem1 dem2 |
DEM1 dem2 |
dem1 DEM2 |
DEM1 DEM2 |
|||
| WT x DEM1/dem1 dem2/dem2a (Col-0) |
0a | n/a | n/a | n/a | n/a | n/a |
| WT x DEM1/dem1 DEM2/dem2 (Col-0) |
58 | 1 (14.5) |
17 (14.5) |
16 (14.5) (20) |
24 (14.5) (20) |
0.0002 0.21 |
| WT x DEM1/dem1 DEM2/dem2 (Ws-0) |
39 | 2 (9.75) |
12 (9.75) |
6 (9.75) (12.5) |
19 (9.75) (12.5) |
0.0007 0.009 |
| WT x DEM1/dem1 DEM2/dem2b (Col-0 and Ws-0) |
97 | 3 (24.25) |
29 (24.25) |
22 (24.25) (32.5) |
43 (24.25) (32.5) |
0.0001 0.009 |
| WT x DEM1/dem1 DEM2/DEM2 (Col-0) |
52 | n/a | n/a | 29 (26) |
23 (26) |
0.41 |
|
DEM1/dem1 dem2/dem2 x WT (Col-0) |
63 | 24 (31.5) |
39 (31.5) |
n/a | n/a |
0.06 |
|
DEM1/dem1 DEM2/dem2 x WT (Col-0) |
48 | 8 (12) |
11 (12) |
9 (12) (14.5) |
20 (12) (14.5) |
0.06 0.04 |
|
DEM1/dem1 DEM2/dem2 x WT (Ws-0) |
24 | 2 (6) |
5 (6) |
6 (6) (8.5) |
11 (6) (8.5) |
0.07 0.23 |
|
DEM1/dem1 DEM2/dem2 x WTb (Col-0 and Ws-0) |
72 | 10 (18) |
16 (18) |
16 (18) (23) |
30 (18) (23) |
0.007 0.04 |
|
DEM1/dem1 DEM2/DEM2 x WT (Col-0) |
33 | n/a | n/a | 16 (16.5) |
17 (16.5) |
0.86 |
a sterile as a male, no progeny was obtained from five separate experiments involving multiple DEM1/dem1 dem2/dem2 plants
bcombined data for Col-0 and Ws-0 backgrounds. n/a, not applicable.