KEY RESOURCES TABLE

REAGENT/RESOURCE	SOURCE	IDENTIFIER
Antibodies
Gamma-tubulin	SigmaAldrich	Cat#T5192
PLK1	Cell Signaling Technology	Cat#4513S
α-tubulin with FITC conjugate	Krackeler Scientific	Cat# 45-T6793
DAPI	SigmaAldrich	Cat#D9542-10MG
NucBlue	ThermoFisher	Cat#R37606
Chemicals, Peptides, and Recombinant Proteins
Agarose	Thermo-Fisher	Cat#16520100
BI2536	Selleck Chemicals	Cat#S1109
BSA	Fisher Scientific	Cat# BP1600-100
Centrinone B	R&D Systems	Cat#5690
Dimethylsulfoxide	Fisher Scientific	Cat#BP231100
GSK461364	Sigma-Aldrich	Cat#45-SML1912-5MG
Paraformaldehyde	Fisher Scientific	Cat#AA433689M
PBS	Fisher Scientific	Cat# 10010023
Prolong Diamond	Fisher Scientific	Cat#P36971
Triton x-100	Fisher Scientific	Cat#BP151500
Tween 20	Thermo-Fisher	Cat# BP337500
Experimental Models: Organisms/Strains
Zebrafish	Zebrafish International Resource Center (ZIRC)	TAB (wild-type)
Zebrafish	Gift from Solnica-Krezel Lab, generated by Harris Lab	Tg(−5actb2:cetn4-GFP)
Zebrafish	Megason Lab	βactin::EMTB-3xGFP aka. Tg(actb2:Hsa.MAP7-EGFP)
C. elegans	Bembenek Lab	JAB23: unc-119(+)]; weIs21[pJA138 (pie-1::mCherry::tub)]
C. elegans	Bembenek Lab	JAB24: zen-4(or153ts); Zen-4:GFP rescue construct complex weIs21 [pJA138 (pie-1::mCherry::tub::pie-1)]
C. elegans	Bembenek Lab	JAB52: unc-119(ed3) iii; ddIs6[tbg-1::GFP + unc- 119(+)] v; ruIs32[Ppie-1::GFP::His-58; unc- 119(ed3) iii; weIs21[pJA138 (pie- 1::mCherry::tub)]
C. elegans	Bembenek Lab	JAB141: ojls2[alpha-tubulin::GFP]; ltIs37 [Ppie-1::mCHERRY::his-58]
C. elegans	Bembenek Lab	JAB142: ojls2[alpha-tubulin::GFP]; ltIs37 [Ppie-1::mCHERRY::his-58]; ltIs44 [Ppie-1::mCherry::PH PLC1delta1]
Software and Algorithms
ImageJ/FIJI	[35]	https://imagej.net/Fiji
IMARIS Bitplane	Oxford Instruments	https://imaris.oxinst.com
Prism8	GraphPad	https://www.graphpad.com/scientific-software/prism/
LAS-X software	Leica Microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/