Table 3.

CRC HAE culture cell yield and success rate depending on the tissue collected

Sampling method	Age group (condition)	Initial cell number	Subculturing method	Success rate in propagation	References
Induced sputum	Children	< 2000 cells/ portion	CRC HAE mono (Rho/SMAD inhibition)	20% in CF patients	Mou et al. [71]
Tracheal aspirate	Neonatal	< 100 cells/ aspirate	CRC HAE mono (Rho/SMAD/mTOR inhibition)	40% from single sample, 80% if multiple samples/ patient	Lu et al. [74]
BAL	Neonatal	< 2000 cells/ portion	CRC HAE, mono (Rho/SMAD inhibition)	100% (multiple samples/ patient)	Mou et al. [71]
Nasal brushing	N/A (CF or healthy)	0.4 to 1.5 million viable cells	Traditional ALI culture	Culture initiation: CF patients—66%; healthy—85% Differentiation in ALI: 100%	Schogler et al. [78]
Bronchial brushing	N/A	0.045 – 0.2 mln cells/ brush	Indirect CRC HAE co-culture: HAE cells cultured with  conditioned fibroblast medium with ROCKi	N/A	Wolf et al. [65]
Bronchial brushing (non-bronchoscopic)	Children (healthy or asthmatic)	~ 2,67 mln cells/ 2 brush passes	CRC HAE co-culture, direct	N/A	Martinovich et al. [50]

BAL Bronchioalveolar lavage, CRC HAE mono conditionally reprogrammed human airway epithelial monoculture (without feeder cells), CRC HAE co-culture conditionally reprogrammed human airway epithelial co-culture (with feeder cells)