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. 2021 May 19;11(5):904. doi: 10.3390/diagnostics11050904

Figure 1.

Figure 1

Schematic overview of sample processing and SARAS-CoV-2 assay workflow depicting main steps: Saliva samples collected in healthcare and community setting were tested and validated as follows: Upper panel: Saliva samples processed with SalivaAll protocol for nucleic acid extraction using a semi-automated instrument, followed by RT-PCR for N, ORF1ab gene targets and IC used as extraction and RT-PCR internal control; Middle panel: Saliva samples processed with SalivaSTAT method that included treatment of samples at 95 °C for 30 min and homogenization using a bead mill homogenizer followed by direct RT-PCR; Lower panel: Saliva samples homogenized using a bead mill before pooling samples with a five-sample pooling strategy followed by SalivaSTAT method for SARS-CoV-2 testing.