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. 2021 May 19;10(10):2191. doi: 10.3390/jcm10102191

Figure 4.

Figure 4

Anchorage-independent three-dimensional spheroid culture enriches HuCCT-1 CSCs. (A) Monolayer culture and 3-D culture of HuCCT-1 cells (scale bar = 200 µm). (BJ) qRT-PCR analysis demonstrated increased expression of embryonic stemness and surface CSC markers in HuCCT-1 spheres compared with HuCCT-1 adherent monolayer culture. Values are mean ± SD of three experiments in triplicate (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ns: not significant). ActB: β-Actin.