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Anchorage-independent three-dimensional spheroid culture of CCLP-1 cells enriches CSCs. (A) Monolayer culture and 3-D culture of CCLP-1 cells (scale bar = 200 µm). (B–I) Enhanced expression of embryonic stemness and cell surface CSC markers were observed in CCLP-1 spheres compared with CCLP-1 adherent monolayer culture by qRT-PCR. Values are mean ± SD of three experiments in triplicate (*** p < 0.005, **** p < 0.001, ns: not significant). ActB: β-Actin.
