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. 2021 May 19;10(10):2191. doi: 10.3390/jcm10102191

Figure 8.

Figure 8

TNF-α-mediated EMT induced upregulation of immune modulators in CCLP-1 cells. (A–F) qRT-PCR showed reduction in the expression of epithelial markers E-Cad, ZO-1 and KRT19, and elevation in the expression of mesenchymal markers N-cad, Fibronectin and ZEB1 after 72 h of TNF-α treatment in CCLP-1 cells. (G–I) qRT-PCR demonstrated elevated expression of immune modulators NT5E and PD-L1 and decreased level of LGALS9 upon 72 h of TNF-α treatment in CCLP-1 cells. Values are mean ± SD of three experiments in triplicate (** p < 0.01, *** p < 0.005, **** p < 0.001, ns: not significant). ActB: β-Actin.