Fig. 4.

The coFrac-MS workflow. Samples are lysed in mild conditions to preserve the integrity of protein complexes, separated under native or near-native conditions using column chromatography or native gel electrophoresis into fractions. Each fraction is then individually subjected to quantitative, bottom-up LC–MS/MS analysis. With the assistance of dedicated computational algorithms, the abundance of each protein is then plotted as co-migration profiles across fractions to construct an interactome network