Fig. 4. Thermal denaturation studies as a measure of thermodynamic stability. (a) CD spectra of NfRedAm as the temperature is increased from 20 to 85 °C. (b) Melting temperatures of AspRedAm, NfRedAm and NfisRedAm. CD220 plotted against temperature to calculate the melting temperatures of (c) AspRedAm and (d) NfRedAm and NfisRedAm.
