Fig. 3. (A) The influence of Nt. BbvCI restriction endonuclease cleaving time (30 min, 60 min, 90 min, 120 min, 150 min) and (B) the incubation time of T7 Exo (30 min, 60 min, 90 min, 120 min, 150 min) on the ECL response based on the proposed biosensor (AFM1 concentration: 0.3 μg mL⁻¹).