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. 2021 May 14;10:e65610. doi: 10.7554/eLife.65610

Table 1. Overview of locations, times, and sample sizes for data acquisition.

Trait category Location Acquisition time
(year, month, duration)
Nesting and intended
total sample size
Realised total sample size Replicates per group
(breeding treatment × sex ×
origin combination)
Reason for sample size reduction
Spatial flower traits (synflorescence height, flower number) Greenhouse and common garden 2019
Jun., Jul., Aug.
5 days, respectively
Two breeding treatments × 2 sexes × 5 maternal families × 8 populations × 2 origins=320 316 36–40 Four individuals died
Flower scent Greenhouse 2019
Jul.
8 hr
Two breeding treatments × 2 sexes × 3 maternal families × 8 populations × 2 origins=192 192 23–35 -
Flower colour Common garden 2019
Aug.
2 weeks
Two breeding treatments × 2 sexes × 5 maternal families × 8 populations × 2 origins=320 286 23–25 Four individuals died, no flowers available for remaining plants
Spatial flower traits (petal limb area and expansion) Common garden 2019
Aug.
2 weeks
Two breeding treatments × 2 sexes × 5 maternal families × 8 populations × 2 origins=320 286 23–35 Four individuals died, no flowers available for remaining plants
Pollinator visitation rates Field site 2020
May–Jul.
8 weeks
Two breeding treatments × 2 sexes × 5 maternal families × 8 populations × 2 origins=320 316 36–40 Four individuals died
Floral rewards Common garden 2020
Aug.
4 weeks
Two breeding treatments × 2 sexes × 5 maternal families × 8 populations × 2 origins=320 280 30–40 Four individuals died, no flowers available for remaining plants