Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus; male/female) |
PvalbCre | The Jackson Laboratory | RRID:IMSR_JAX:008069 | C57BL/6J background |
| Genetic reagent (M. musculus; male/female) |
Slc32a1Cre | The Jackson Laboratory | RRID:IMSR_JAX:028862 | C57BL/6J background |
| Genetic reagent (M. musculus; male/female) |
Slc17a6Cre | The Jackson Laboratory | RRID:IMSR_JAX:028863 | C57BL/6J background |
| Genetic reagent (M. musculus; male/female) |
LeprCre | M.G. Myers Jr., University of Michigan Medical School | RRID:IMSR_JAX:032457 | C57BL/6J background |
| Antibody | Anti-DsRed, rabbit polyclonal | Takara Bio, Inc | Cat # 632496 RRID:AB_10013483 |
(1:1000) |
| Antibody | Anti-parvalbumin (PVALB), guinea pig polyclonal | Swant | Cat # GP72; RRID:AB_2665495 | (1:300) |
| Antibody | Anti-rabbit Alexa Fluor 488, goat polyclonal | Thermo Fisher Scientific | Cat # A11034 RRID:AB_2576217 |
(1:500) |
| Antibody | Anti-guinea pig Alexa Fluor 488, donkey polyclonal | Jackson ImmunoResearch Laboratories | Cat # 706-545-148; RRID:AB_2340472 | (1:500) |
| Antibody | Anti-guinea pig Alexa Fluor 647, donkey polyclonal | Jackson ImmunoResearch Laboratories | Cat # 706-605-148; RRID:AB_2340476 | (1:500) |
| Recombinant DNA reagent | rAAV2/9-CAG-FLEX-GCaMP6s-WPRE-SV40 | Addgene | RRID:Addgene_10084; Addgene viral prep 100842-AAV9 | 5.0 × 1012 GC/ml |
| Recombinant DNA reagent | rAAV2/rh10-hSYN-DIO-hM3D(Gq)-mCherry | University of North Carolina (UNC) Vector Core | RRID:Addgene_44361 | 2.0 × 1012 GC/ml |
| Recombinant DNA reagent | rAAV2/rh10-hSYN-DIO-hM4D(Gi)-mCherry | UNC Vector Core | RRID:Addgene_44362 | 2.0 × 1012 GC/ml |
| Recombinant DNA reagent | rAAV2/9-hSYN-DIO-mCherry | Addgene | RRID:Addgene_50459; Addgene viral prep 50459-AAV9 | 2.1 × 1013 GC/ml |
| Recombinant DNA reagent | rAAV2/1-CAG-FLEX-rev-ChR2-tdTomato | Addgene | RRID:Addgene_18917; Addgene viral prep 18917-AAV1 | 6.9 × 1012 GC/ml |
| Recombinant DNA reagent | rAAV2/9-CAG-FLEX-ArchT-GFP | UNC Vector Core | RRID:Addgene_29777 | 4.7 × 1012 GC/ml |
| Recombinant DNA reagent | rAAV2/9-CAG-FLEX-GFP | University of Pennsylvania (U Penn) Vector Core | RRID:Addgene_51502 | 3.3 × 1013 GC/ml |
| Recombinant DNA reagent | rAAV2/1-CAG-FLEX-tdTomato | U Penn Vector Core | RRID:Addgene_51503 | 4.5 × 1013 GC/ml |
| Recombinant DNA reagent | rAAV2/9-CAG-FLEX-tdTomato | U Penn Vector Core | RRID:Addgene_51503 | 4.1 × 1013 GC/ml |
| Recombinant DNA reagent | rAAV2/8-hSYN-FLEX-TVA-Rabies B19G (TVA+) | Michigan Diabetes Research Center Molecular Genetics Core, University of Michigan | 4 × 1012 GC/ml | |
| Recombinant DNA reagent | EnvA-∆G-Rabies-mCherry | Michigan Diabetes Research Center Molecular Genetics Core, University of Michigan | 1 × 1010 pfu/ml | |
| Chemical compound, drug | Clozapine N-oxide (CNO) | Tocris Bioscience | Cat # 4936; PUBCHEM:135445691 | |
| Chemical compound, drug | Acetic acid | Sigma-Aldrich | Cat # 320099; PUBCHEM:176 | |
| Chemical compound, drug | Formalin | Macron Fine Chemicals | Cat # 5016–02; PUBCHEM:712 | |
| Chemical compound, drug | Complete Freund’s adjuvant (CFA) | Sigma-Aldrich | Cat # F5881 | |
| Chemical compound, drug | Morphine | National Institute on Drug Abuse Drug Supply Program | PUBCHEM:5288826 | |
| Software, algorithm | ANY-maze video tracking system v5 | Stoelting Co. | RRID:SCR_014289 | |
| Software, algorithm | Doric Neuroscience Studio v5.1 | Doric Lenses Inc | RRID:SCR_018569 | |
| Software, algorithm | FIJI/ImageJ v1.52p | https://imagej.net/Fiji | RRID:SCR_002285 | |
| Software, algorithm | Prism 8 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | Miniscope Analysis Pipeline | Etter, 2021 | ||
| Software, algorithm | CellReg | Sheintuch et al., 2021 | ||
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | R2019a & R2020a |
| Other | Snap-in Imaging Cannula Model L-V | Doric Lenses Inc | GRIN lenses | |
| Other | Basic Fluorescence Snap-In Microscopy System – Deep Brain | Doric Lenses Inc | In vivo imaging system |