Fig. 1. Comparison of protein structures of β-gal from different species. (a) Superimposition of the catalytic domains of E. coli β-gal and human β-gal (upper). Superimposition of the catalytic domains of A. oryzae β-gal and human β-gal (lower). (b) Shape I: shape of the binding pocket of human β-gal with galactose; Shape II: shape of the binding pocket of E. coli β-gal with galactose. The Ms volume, pocket Ms area, and mouth Ms area for human β-gal vs. E. coli β-gal are 457.2 Å3vs. 280.1 Å3, 231.1 Å2vs. 261.2 Å2, 86.4 Å2vs. 41.0 Å2, respectively. The pockets were analysed by CASTP.34.
