Summary of the photophysical properties of FC1–FC11 and R1–R3 in toluene and 10% FBS.
| Entry | λ abs max (nm) | λ em max (nm) | ε × 103 (M−1 cm−1) | Φ F b |
|---|---|---|---|---|
| [Tol/FBS]a | [Tol/FBS]a | [Tol/FBS]a | [Tol] | |
| FC1 | 380/467 | 489/578 | 8.0/7.2 | 0.54 |
| FC2 | 461/491 | 565/575 | 12/11 | 0.74 |
| FC3 | 483/500 | 567/572 | 14/13 | 0.81 |
| FC4 | 493/514 | 597/598 | 13/12 | 0.59 |
| FC5 | 470/480 | 570/572 | 8.7/7.8 | 0.75 |
| FC6 | 530/560 | 636/630 | 12/5.6 | 0.55 |
| FC7 | 469/480 | 580/565 | 13/15 | 0.46 |
| FC8 | 596/625 | 740/735 | 9.2/8.9 | 0.32 |
| FC9 | 602/608 | 737/725 | 8.9/7.7 | 0.23 |
| FC10 | 668/694 | 770/807 | 11/6.6 | 0.09 |
| FC11 | 408/422 | 551/531 | 14/7.9 | 0.57 |
| R1 | 362/381 | 417/443 | 25/20 | 0.81 (ref. 45) |
| R2 | 371/382 | 419/445 | 25/23 | 0.88 |
| R3 | 466/485 | 523/520 | 32/30 | 0.03 |
a
Optical properties were measured in toluene (Tol) and 10% FBS in pH 7.4 PBS.
b
Absolute ΦF were measured using an integrating sphere.32 See Table S1 and Section S3 for additional data and discussion.