Fig. 3. (A) Tau (0N4R) protein were collected in 100 μL aliquots every 24 hours from the aggregation reaction and then stored at −80 °C after flash freezing. After 240 hours of incubation 1 mL of tau aggregates was collected and loaded on the top of the ultracentrifuge. (B–G) TEM images of tau aggregates structures at fractions from 50% to 5% sucrose gradient after 4 hours of ultracentrifugation (white bar = 500 nm).