Binding affinity of pTP-TFE and pFTAA with 10% to 50% tau fraction (n = 3–6) and Aβ40 aggregates at 90 min, 200 min and 24 hours (n = 3) after ultracentrifugation, Kd values were fitted using GraphPad Prism5.
| Aggregates | pTP-TFE, Kd (μM) | pTP-TFE, binding ratioa | pFTAA, Kd (μM) | pFTAA, binding ratiob |
|---|---|---|---|---|
| 10% Sucrose tau | 0.96 ± 0.20 | 14 | 29.57 ± 2.10 | 148 |
| 20% Sucrose tau | 0.07 ± 0.03 | 1 | 2.75 ± 0.57 | 14 |
| 30% Sucrose tau | 3.04 ± 0.71 | 49 | 5.27 ± 1.25 | 26 |
| 40% Sucrose tau | 0.59 ± 0.20 | 9 | 1.29 ± 0.20 | 6 |
| 50% Sucrose tau | 1.08 ± 0.17 | 15 | 0.20 ± 0.01 | 1 |
| 90 Minute, Aβ | 7.58 ± 2.47 | 108 | No affinity | N/A |
| 200 Minute, Aβ | 3.27 ± 0.64 | 47 | 0.93 ± 0.30 | 5 |
| 24 Hour, Aβ | 3.79 ± 0.34 | 54 | 0.65 ± 0.19 | 3 |
a
Binding ratio is based on comparison of Kd values with the 20% sucrose tau Kd.
b
Binding ratio is based on comparison of Kd values with the 50% sucrose tau Kd.