Fig. 4. Analysis of total IgG antibodies elicited against the synthetic glycodendrimers. Microtiter plates were coated with the four different glycosylated scaffolds lacking the OVA epitopes: (a) 8, (b) 5, (c) 6, and (d) 2 (see Scheme 1); and the antisera from mice A to E were probed. Statistical significance compared to no-adjuvant control group (Group E) was assessed using two-tailed unpaired Student's t-test: ***p < 0.001.