Fig. 6. Cell surface reactivity of mouse antisera against native Tn antigen-expressed on the surface of human breast cancer cell line, MCF7. MCF7 cells were incubated with antisera from mice of Group A and IgG antibody binding was analyzed by indirect immunofluorescence. (a) Fluorescence images of positive control (Ct+), sera from non-immunized mice (unimmunized), sera from no-adjuvant control Group E and sera from Group A mice, respectively; scale bar = 50 × 10−6 m. (b) Detailed region of interest (ROI) of positive control and Group A sera; scale bar = 10 × 10−6 m.
