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. 2021 Jun 1;131(11):e143377. doi: 10.1172/JCI143377

Figure 8. CD147, ICAM-1, EGFR, and EPHA2 are mediators of FUT1 that regulate cancer stemness via the AKT/mTOR/4EBP1 signaling axis.

Figure 8

(A) Schematic representation of our experimental approach to fucosylated-peptide profiling. (B) Venn diagram showing the number of proteins identified by nano–LC MS/MS analysis of the UEA-1–enriched fractions of cells treated with empty vector control and cells with FUT1 overexpression, as well as the common proteins that matched the Cancer Stem Cell database (CSCdb; ref. 15). Of the 30 proteins, 24 are related to PI3K/AKT signaling, including 4 cell surface proteins (ICAM1, CD147, EGFR, and EPHA2). (C) UEA-1 affinity chromatography of whole-cell lysates of Huh7 HCC cells transfected with empty vector or with FUT1 overexpression and in the absence or presence of 2DGal or Huh7 HCC cells cultured in high or low glucose, followed by Western blotting with LAMP2, CD147, ICAM-1, EGFR, and EPHA2 antibodies. Input shows no effect of glucose restriction and FUT1 overexpression on protein expression. LAMP2 was used as positive control. The data shown are representative of 3 independent experiments. EV, empty vector control; OE, overexpression; HG, high glucose; LG, low glucose; 2DGal, 2-deoxy-D-galactose.