Figure 4. SIGLEC-3 mediates the hHBV immunosuppressive effect.
(A and B) hHBV virion inhibited cytokine secretion in TLR ligand–stimulated moDCs. MoDCs (6 × 104 per well) pretreated with hHBV or mHBV in different doses for 24 hours before incubation with Pam3csk4 or poly (I:C) and IFN-γ for another 24 hours. Cytokine levels in the supernatant were determined by ELISA. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (2-way ANOVA) (n = 3–4). (C) Recruitment of SHP-1 and SHP-2 by hHBV is time dependent. MoDCs were treated with hHBV (109 copies/mL) to observe recruitment of SHP-1 and SHP-2 in moDCs by Western blotting. Signals were quantified by densitometry using ImageJ software. The values represent the fold-change normalized to the starting point. Experiments were performed and repeated 3 times with similar results.