Figure 5. CsA inhibits perforin expression more potently in Lck^WT T cells.

(A–C) Freshly isolated liver-infiltrating cells from recipients receiving B6 WT (n = 6), WT + CsA (n = 5), Lck^S59A (n = 5), or Lck^S59A + CsA (n = 5) donors were stained for CD107a (A), granzyme B (B), and perforin (C) expression. The cells were analyzed by flow cytometry and gated on CD8⁺. Because of limiting cell numbers, the negative control is TCR-β⁺–gated splenocytes from mice that received BM only, and is included with the histograms of both WT and Lck^S59A CD8⁺ cells. The error bars represent the mean ± SEM. (D) Flow cytometric analysis of perforin staining is shown for CD8⁺-gated P14 T cells stimulated on anti-CD3 coated plates and soluble anti-CD28 for 48 hours. The graphs show the relative MFIs of perforin expression compared with activated and CsA-untreated CD8⁺ T cells. The graphs show the mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01, 2-tailed Student’s t test.