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. 2021 Jun 1;131(11):e146987. doi: 10.1172/JCI146987

Figure 13. Hgf promotes tumor cell proliferation through Met receptor.

Figure 13

(A) Representative IHC images and quantification of Ki67+ cells in Pan02 and CMT93 models in Hgffl/fl and HgfΔHSC animals (n = 10–15 per group). Scale bars: 100 μm. (B) Pan02 cells treated with recombinant mouse HGF (25 ng/ml) or vehicle for 10 minutes followed by a phospho-kinase array and Western blot for phosphorylated and total ERK1/2 and AKT. (C) Pan02 cells treated with HGF (25 ng/ml) or Merestinib (3 mM) or U0126 (10 mM) and followed by recombinant mouse HGF (25 ng/ml) or vehicle for 48 hours followed by Ki67 staining. Scale bars: 250 μm. Statistics were done using Mann Whitney U test or unpaired t test dependent on data distribution (A) or 1-way ANOVA with Kruskal Wallis post hoc test (C). Data are shown as the mean ± SEM.