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. 2021 Jun 1;131(11):e146987. doi: 10.1172/JCI146987

Figure 3. HSC-derived CAF promote desmoplastic tumor growth and mortality.

Figure 3

(A and B) HSC-CAF depletion via DT injection (0.5 ng/g BW) in LratCre+TdTom+iDTR+ or LratCre+TdTom+iDTR– littermates (B) reduces TdTom expression and Sirius red in tumors. Scale bars: 100 μm. (C) Representative macroscopic and IHC images of liver and liver-to-body weight ratio (LBR) and tumor area quantifications (n = 7–15 mice/group), showing effects of HSC-CAF depletion on desmoplastic (Pan02, CMT93) but not on nondesmoplastic (EO771, B16F10) liver metastasis. Scale bars: 1 cm. (D) Survival studies in iDTR mice injected with DT until death (n = 5–8 mice/group for each model). Statistics were done by 2-tailed unpaired t test or Mann Whitney U (B and C) or Mantel-Cox test (D). Data are shown as the mean ± SEM.