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. 2021 Jun 1;131(11):e146987. doi: 10.1172/JCI146987

Figure 5. CAF depletion in advanced stages arrests growth and promotes survival.

Figure 5

(A) HSC-CAF depletion via DT injection (0.5 ng/g) in LratCre+TdTom+iDTR+ or LratCre+TdTom+iDTR– littermates. (B and C) Representative macroscopic and IHC images of liver and liver-to-body weight ratio (LBR) and tumor area quantification (n = 6–8 mice/group), showing the effects of late HSC-CAF depletion on desmoplastic tumors (Pan02, CMT93). Scale bars: 1 cm. (D) Survival studies in iDTR mice injected with DT (n = 5 mice/group). DT was injected from day 13 onward at intervals of 3 days until the end of the study. (E) Contrast-CT scan analysis and tumor volume quantification of animals undergoing late HSC-CAF depletion via LratCre × iDTR. Arrows indicate metastatic tumors (dark areas); lighter areas show nontumor tissue. Statistics were done by 2-tailed unpaired t test or Mann Whitney U dependent on data distribution (B, C, and E) or Mantel-Cox test (D). Data are shown as the mean ± SEM.