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. 2020 Feb 13;26(5):1458–1471. doi: 10.1038/s41380-020-0681-0

Fig. 4. Dysregulated Pten expression in forebrains of Ptenm3m4/m3m4 mice correlates with decreases in synaptic marker expression and increased microglial activation in vivo.

Fig. 4

a Expression of Pten in the forebrain of PtenWT/WT, PtenWT/m3m4, and Ptenm3m4/m3m4 mice quantified by western blot analyses at P2, P8, P14, P21, and P40; P2 (relative expression = 0.63; H = 11.92, p value = 0.002), P8 (relative expression = 0.52; H = 11.70, p value = 0.002), P14 (relative expression = 0.69; H = 7.98, p value = 0.01), P21 (relative expression = 0.41; H = 14.24, p value = 0.0005), P40 (relative expression = 0.36; H = 12.44, p value = 0.001). b Expression of Syn in the forebrain of PtenWT/WT, PtenWT/m3m4, and Ptenm3m4/m3m4 mice quantified by western blot analyses at P2, P8, P14, P21, and P40; P40 (relative expression = 0.62; H = 10.43, p value = 0.02). c Expression of Psd-95 in the forebrain of PtenWT/WT, PtenWT/m3m4, and Ptenm3m4/m3m4 mice quantified by western blot analyses at P2, P8, P14, P21, and P40; P40 (relative expression = 0.60; H = 8.6, p value = 0.01). d Immunofluorescent staining for Syn (green) and Iba1 (red) in PtenWT/WT, PtenWT/m3m4, and Ptenm3m4/m3m4 CA3 hippocampal regions at P14 and P40. e Quantification of Syn in CA3 region of the hippocampus at P14 (mean integrated density of Syn: PtenWT/WT = 1.06 ± 0.13; PtenWT/m3m4 = 1.15 ± 0.46; F = 7.17, p value = 0.02; Ptenm3m4/m3m4 = 1.85 ± 0.41; F = 7.17, p value = 0.01) and P40 (mean integrated density Syn: PtenWT/WT = 0.96 ± 0.33; Ptenm3m4/m3m = 0.47 ± 0.04; F = 4.9, p value = 0.03), as well as microglia cell area P40 (mean Iba1 area:  PtenWT/WT = 3.62 ± 0.89; Ptenm3m4/m3m4 = 10.41 ± 1.70; F = 12.5, p value = 0.0009).