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. 2020 Apr 23;26(5):1606–1618. doi: 10.1038/s41380-020-0728-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Semi-quantitative RT-PCR analysis of PTBP2 exon 10 splicing in undifferentiated or differentiated SH-SY5Y cells transfected with siRNAs (n = 4; Mann–Whitney test: *p < 0.05). b Representative western blot of MOCOS and PTBP2 expression and c quantification of PTBP2 protein level at 5DIV in undifferentiated and differentiated neuroblastoma cells. Cells were depleted in COSMOC or MOCOS expression and compared with cells treated with control siRNA. GAPDH was used as control. (n = 4; Mann–Whitney test: *p < 0.05). d Semi-quantitative RT-PCR analysis of PSD95 exon 18 splicing in undifferentiated or differentiated SH-SY5Y cells treated with siRNAs and assessed at 5DIV. (n = 4; Mann–Whitney test: *p < 0.05). e Agarose gels and f quantification of RT-PCR products of PTBP2 exon 10 inclusion. RNA was isolated from OSCs of ASD patients (from A1 to A11) and OSCs of their matched healthy individuals (from C1 to C11) (n = 11; Mann–Whitney test: **p < 0.01). Data are represented as mean ± SEM. For all graphs, n stands for the number of biological replicates of SH-SY5Y cells or OSC.