Table 4.

Effects of SCE, neosalacinol (4), and voglibose on food and water intakes, body weight, and HbA1c levels after 20 days of administration in AIN-93 M purified diet-fed ob/ob mice

	Dose	Food intake	Water intake	Body weight (g)
	(%)	(g/day, average)		Day 0	Day 3	Day 6	Day 9	Day 12	Day 15	Day 18	Day 20
Control	–	3.5 ± 0.1	4.0 ± 0.7	32.9 ± 0.6	35.6 ± 0.7	36.4 ± 0.4	36.9 ± 0.5	38.1 ± 0.4	39.2 ± 0.5	40.3 ± 0.4	41.0 ± 0.4
SCE	0.05	3.6 ± 0.1	2.5 ± 0.1**	33.4 ± 0.5	36.1 ± 0.3	36.8 ± 0.2	37.4 ± 0.2	38.6 ± 0.3	39.3 ± 0.2	40.8 ± 0.3	41.3 ± 0.3
Neokotalanol (4)	0.0003	3.5 ± 0.2	2.5 ± 0.2**	32.2 ± 0.5	35.1 ± 0.4	35.8 ± 0.4	36.0 ± 0.4	37.2 ± 0.4	37.8 ± 0.4	39.4 ± 0.4	40.1 ± 0.4
Voglibose	0.0001	4.5 ± 0.2	3.3 ± 0.2	33.0 ± 0.5	36.2 ± 0.4	36.3 ± 0.5	37.1 ± 0.3	38.0 ± 0.4	39.0 ± 0.2	40.2 ± 0.3	40.8 ± 0.4

	Dose	HbA1c (%)
	(%)	Day 0	Day 20
Control	–	6.1 ± 0.0	6.5 ± 0.2
SCE	0.05	6.1 ± 0.1	6.0 ± 0.1*
Neokotalanol (4)	0.0003	5.9 ± 0.1	5.9 ± 0.1**
Voglibose	0.0001	5.9 ± 0.1	6.0 ± 0.1*

Effects on HbA1c levels of AIN-93 M purified diet-fed ob/ob mice following 20 days of administration: Male B6.Cg-Lep^ob/J (ob/ob) mice (6-week-old, Charles River Laboratories Japan, Inc., Yokohama, Japan) were housed for 1 week in individual meal cages. These mice were divided into four groups based on body weight, blood glucose, and HbA1c levels. Mice in the control group was fed a standard AIN-93 M purified diet. Mice in the SCE-, neokotalanol (4)-, and voglibose-treated groups were fed the same diet supplemented with 0.05, 0.0003, and 0.0001% (w/w) of the respective treatments. On days 0, 3, 6, 9, 12, 15, 18, and 20 (end of the treatment period), their body weights were measured. On days 0 and 20, the HbA1c levels were measured using Quo-Lab (Nipro, Osaka, Japan)

Each value represents the mean ± S.E.M. (n = 6)

Significantly different from the control: *p < 0.05, **p < 0.01

Reproduced with permission from J. Nat. Med., 73, 584–588. Copyright [2019] Springer Nature