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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Acta Physiol (Oxf). 2021 Mar 11;232(1):e13640. doi: 10.1111/apha.13640

Figure 3. Renal expression and urinary excretion of prostasin in Prss8-wt, Prss8-S238A and Prss8-R44Q mutant mice.

Figure 3.

A Western blot appearance of wild-type and mutant prostasin in kidney tissue as well as a recombinant truncated murine prostasin (amino acids 30-289, predicted mass 28 kDa) with or without deglycosylation by PNGaseF. Note that deglycosylation leads to single clear bands, compatible with glycosylation of prostasin in vivo.

B Expression of prostasin as analyzed by Western blot from kidney lysates treated with PNGaseF for deglycosylation. Representative lanes with n=3 for each genotype.

C Densitometric analysis of the obtained bands from two Western blots with n=5-6 for each genotype.

D Urinary excretion of prostasin measured with an ELISA in 24 h urine samples.