Table 1:
Plasma values of Prss8-wt, Prss8-R44Q and Prss8-S238A mutant mice before and after triamterene treatment
| control | + triamterene | |||||
|---|---|---|---|---|---|---|
| Prss8-wt | Prss8 R44Q |
Prss8 S238A |
Prss8-wt | Prss8-R44Q | Prss8-S238A | |
| N | 9-13 | 5-7 | 6 | 21-24 | 12-16 | 12-14 |
| Na+ [mmol L−1] | 150 ± 1 | 150 ± 1 | 149 ± 0.4 | 149 ± 1 | 156 ± 3 * | 148 ± 2 |
| K+ [mmol L−1] | 4.3 ± 0.1 | 3.8 ± 0.1* | 4.4 ± 0.1 | 5.5± 0.3 # | 6.6 ± 0.5 # | 5.6 ± 0.4 # |
| pH | 7.29 ± 0.01 | 7.21 ± 0.02 * | 7.29 ± 0.01 | 7.17 ± 0.02 # | 7.01 ± 0.03 #* | 7.20 ± 0.03 |
| pCO2 [mmHg] | 45 ± 2 | 56 ± 3 * | 46 ± 2 | 52 ± 2 # | 66 ± 3 * | 52 ± 1 # |
| std HCO3− [mmol L−1] | 20 ±0.5 | 21 ± 1 | 21 ± 0.5 | 16 ± 1 # | 12 ± 1 #* | 18 ± 1 |
| hematocrit [%] | 46 ± 1 | 45 ± 1 | 47 ± 1 | 56 ± 1 # | 57 ± 1 #* | 52 ± 1 * # |
| urea [mg dL−1] | 44 ± 6 | 52 ± 9 | 40 ± 10 | 96 ± 14 # | 199 ± 26 #* | 111 ± 18 # |
| aldosterone [pg mL−1] | 225 ± 39 | 339 ± 118 | 185 ± 42 | 1673 ± 263 # | 3767 ± 852 #* | 1597 ± 344 # |
Arithmetic means ± SEM from venous blood gas analysis (under isoflurane narcosis) and direct determination (urea, aldosterone) before and at the end of the 4 day treatment with triamterene. In some of the Prss8-R44Q mice, blood was collected before premature termination of the experiments after the 2nd or 3rd day of triamterene treatment. Std HCO3− denotes HCO3− normalized for a pCO2 of 40 mm Hg.
#
indicates significant difference between control and triamterene treatment
*
indicates significant difference between the genotypes