Extended Data Fig. 8. mTOR and protein synthesis regulate ferroptosis.

a, Phosphorylation and levels of mTOR pathway effectors in U-2 OS cells. Blot is representative of three independent experiments. b, Analysis of Cancer Therapeutics Response Portal (CTRP) dataset for ferroptosis-inducing compounds. c, Cell death determined using STACK. Erastin2 was used at 2 μM in all cell lines except Caki-1^N (1 μM), ML162 was used at 4 μM in all cell lines except Caki-1^N (2 μM). CHX: cycloheximide, BSO: buthionine sulfoximine. Results are mean ± SD from three independent experiments.