Figure 6. Myeloid cell-targeted ADAM17-deficiency selectively reduces the concentration of large HDL particles in APOA1^Tg; Ldlr^−/− mice.

Bone marrow from myeloid cell-targeted ADAM17-deficient mice or C57BL/6J WT controls was transplanted into lethally irradiated (10 Gy) male Ldlr^−/− or APOA1^Tg; Ldlr^−/− recipient littermates. After 8 weeks of recovery, collected plasma samples were used to measure HDL particle concentration and size by calibrated ion mobility analysis. A. Representative size distributions of small, medium, and large HDL from Ldlr^−/− and APOA1^Tg; Ldlr^−/− recipients. B. Total HDL particle concentration (n=5). C. Small (S-HDL), medium (M-HDL) and large HDL (L-HDL) particle concentration (n=5). Data are shown as mean ± SEM. Tests for normality (Shapiro-Wilk) and equal variance (Brown-Forsythe) were performed for each of the data sets. P values were determined accordingly by one-way ANOVA followed by Tukey’s multiple comparison tests (B) and two-way ANOVA followed by Sidak’s multiple comparison tests (C).